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Ec_16S-PKcentre

PKB-number: PKB64
Definition: The central pseudoknot of 16S ribosomal RNA of E.coli
Organism: E.coli
Abbreviation: Ec_16S-PKcentre
RNA type: rRNA
Keywords: ribosome; translation; rRNA
EMBL number: E05133
Submitted by: A.P.Gultyaev ( A.P.Gultyaev@Biology.LeidenUniv.nl)
Supported by: Mutagenesis; Sequence comparison
References: [1] Pleij C.W.A. et al. (1985). Nucleic Acids Res. 13:1717-1731.
[2] Poot R.A. et al. (1998). Nucleic Acids Res. 26:549-553.
[3] Gutell R.R. (1993). Curr.Opin.Struct.Biol. 3:313-322.
[4] Van de Peer Y. et al. (1999). Nucleic Acids Res. 27:179-183. (database: http://rrna.uia.ac.be/ssu/)
Comment: Initially suggested on the basis of phylogenetic comparisons [1], the central pseudoknot was shown to be important for ribosome functioning by mutagenesis [2]. The pseudoknot is also conserved in other 16S-like rRNAs: for review see e.g.[3], database on the small subunit ribosomal RNA [4].
Stem sizes:
Loop sizes:
5 3
3 1 890
Position Paired:
9-13; 21-25
17-19; 916-918
Bracket view of structure:
                 10        20
     #   123456789|123456789|123456(888)
     $ 1 AAAUUGAAGAGUUUGAUCAUGGCUCA=26
     % 1 ::::::::(((((:::[[[:))))):
              920
     #(888)56789|
     $ 915 AUGAAU=920
     % 915 :]]]::

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© final design and maintenance: F.H.D.(Eke) van Batenburg; 1999-6-1 PKB00064.html